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Primer

Part:BBa_K2110821:Design

Designed by: Haipeng Yu   Group: iGEM16_Tianjin   (2016-10-05)


PETase site mutation W159A - F


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

First apply PCR for mutant section. Then PCR again with original primers of PETase to overlap for the entire mutant PETase.


Source

Artificially Designed

References